# Protein Iso electric Point Calculation – Henderson Equation

Henderson equation is an approximate equation that shows the relationship between the pH or pOH of a solution and the pKa or pKb and the ratio of the concentrations of the dissociated chemical species. Virginia Henderson, FRCN (November 30, 1897-March 19, 1996) was a nurse, researcher, theorist and author. Use this calculator to find the iso electric points of proteins by using the Henderson equation.
Henderson calculator
 HCO3 (mEq/L): PaCO2 (mmHg): pH Value = (Unit)
Formula
Henderson Hasselbalch Equation = 6.1 + log ((HCO3)/ (0.0301*PaCo2))
The isoelectric point (pI), sometimes abbreviated to IEP, is the pH at which a particular molecule or surface carries no net electrical charge.

Example:
Calculate the iso electric points of the proteins by the given details.
HCO3 (mEq/L) = 25
PaCO2 (mmHg) = 30

Solution:
Apply Formula:
Henderson Hasselbalch Equation = 6.1 + log ((HCO3)/ (0.0301*PaCo2))

pH Value = 9.420908550433353 (unit)